aβ nab228  (Santa Cruz Biotechnology)

Journal: Acta Neuropathologica Communications

Article Title: Cryo-EM structures of Aβ40 filaments from the leptomeninges of individuals with Alzheimer’s disease and cerebral amyloid angiopathy

doi: 10.1186/s40478-023-01694-8

Figure Lengend Snippet: Aβ immunoreactivity of leptomeningeal blood vessels. Anti-Aβ antibody NAB228 was used to stain the leptomeninges from Alzheimer’s disease cases 1 ( a ), 2 ( b ) and 3 ( c ). Scale bar, 200 μm

Article Snippet: Immunohistochemistry using anti-Aβ antibody NAB228 (1:200, Invitrogen) was carried out as described [ ].

Techniques: Staining

Journal: Diagnostics

Article Title: Development and Implementation of an Internal Quality Control Sample to Standardize Oligomer-Based Diagnostics of Alzheimer’s Disease

doi: 10.3390/diagnostics13101702

Figure Lengend Snippet: Comparison of several Aβ oligomer quantification assays according to their sensitivity level. For this purpose, the respective weight concentrations pg/mL were converted into femtomolar oligomer concentrations using the approximate molecular weights of the used calibration standards.

Article Snippet: The anti-Aβ antibody IC16 (mouse, monoclonal, amino acids (aa)2–8, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany) labeled with CF633 dye (Biotium, Freemont, CA, USA) and the anti-Aβ antibody Nab228 (mouse, monoclonal, aa1–11, Sigma-Aldrich) labeled with CF488A dye (Biotium) were used as detection probes for TIRFM.

Techniques: Single Particle, Fluorescence, Microscopy, Bead-based Assay, Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Beneficial Effect of ACI-24 Vaccination on Aβ Plaque Pathology and Microglial Phenotypes in an Amyloidosis Mouse Model.

doi: 10.3390/cells12010079

Figure Lengend Snippet: Figure 1. ACI-24 vaccination efficiently reduces Aβ plaque load in APPPS1 mice. Schematic overview of the vaccination paradigm (A). ELISA analysis of the anti-Aβ42 total IgG titres (ng/mL) in plasma (B). Control APPPS1 mice (PBS treated, in dotted grey) have the baseline titres, while both ACI-24 vaccinated APPPS1 (in red) and ACI-24 vaccinated WT mice (in black) have robust and sus- tained immune response. Representative images of Aβ (as stained with NAB228 antibody) and nuclei (Hoechst) in cortical sections show decreased Aβ deposition following ACI-24 vaccination. Scale bar for images in the centre column is indicating 200 µm, and scale bar for images in the right column (magnification of dotted boxed regions from centre column images) is indicating 50 µm (C). Statistical analysis of 10 control and 10 ACI-24 vaccinated APPPS1 mice for Aβ plaque coverage (D), number (E) and size (F). ACI-24 significantly downregulated Aβ plaque coverage and size. Offspring are from the transgenic mother (pink circles) or father (blue circles). Graphs are presented as mean ± SEM (* p < 0.05, ** p < 0.001, unpaired two-tailed Student’s t-test).

Article Snippet: Cells 2023, 12, 79 6 of 19 Primary antibodies used: Aβ 3552 (0.74 μg/mL) [49], Aβ NAB228 (1:500, sc-32277, Santa Cruz Biotechnology, Heidelberg, Germany) [10], BACE1 (1:400, ab108394, Abcam, Cambridge, UK), ApoE (1:200, hybridoma supernatant, clone 26C11), IBA1 (1:500, ab5076, Abcam, Cambridge, UK), IBA1 (1:500, 234308, Synaptic Systems, Göttingen, Germany), NLRP3 (1:50, BSS-BS-10021R-100, Biozol Diagnostics Vertrieb, Eching, Germany) and CD68 (1:500, MCA1957GA, Bio-Rad, Feldkirchen, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Control, Staining, Transgenic Assay, Two Tailed Test

Journal: Cells

Article Title: Beneficial Effect of ACI-24 Vaccination on Aβ Plaque Pathology and Microglial Phenotypes in an Amyloidosis Mouse Model.

doi: 10.3390/cells12010079

Figure Lengend Snippet: Figure 6. ACI-24 reduces ApoE protein levels and neuronal injury in the offspring generated by the transgenic mother. Representative images of control and ACI-24 vaccinated mice stained for nuclei (Hoechst), fibrillar Aβ (ThR), Aβ plaques (3552 antibody) and ApoE (A); and nuclei (Hoechst), fibrillar Aβ (ThR), Aβ plaques (NAB228 antibody) and BACE1 (dystrophic neurites) (B). Scale bar: 200 µm. Statistical analysis of 5 control and 6 ACI-24 vaccinated mice (transgenic mother offspring) showing significant downregulation of both total ApoE (C) and BACE1 (D) coverage. Graphs are presented as mean ± SEM (* p < 0.05, ** p < 0.001, unpaired two-tailed Student’s t-test).

Article Snippet: Cells 2023, 12, 79 6 of 19 Primary antibodies used: Aβ 3552 (0.74 μg/mL) [49], Aβ NAB228 (1:500, sc-32277, Santa Cruz Biotechnology, Heidelberg, Germany) [10], BACE1 (1:400, ab108394, Abcam, Cambridge, UK), ApoE (1:200, hybridoma supernatant, clone 26C11), IBA1 (1:500, ab5076, Abcam, Cambridge, UK), IBA1 (1:500, 234308, Synaptic Systems, Göttingen, Germany), NLRP3 (1:50, BSS-BS-10021R-100, Biozol Diagnostics Vertrieb, Eching, Germany) and CD68 (1:500, MCA1957GA, Bio-Rad, Feldkirchen, Germany).

Techniques: Generated, Transgenic Assay, Control, Staining, Two Tailed Test